SynNotch-CAR T cells overcome challenges of specificity, heterogeneity and persistence in treating xenograft model of glioblastoma.
Science Translational Medicine. 2021,PDF. PRESS STAT. PRESS UCSF.
T cells selectively filter oscillatory signals on the minutes timescale.
Proceedings of the National Academy of Sciences. 2021, March 2PDF.
T cell circuits that sense antigen density with an ultrasensitive threshold.
Science. 2021PDF. FEATURED IN Cell Research. FEATURED IN Signal Transduction and Targeted Therapy
DNA scaffolds enable efficient and tunable functionalization of biomaterials for immune cell modulation.
Nature Nanotechnology. 2020PDF.
Engineering cytokines and cytokine circuits: Learning to speak the secret language of immune cells could improve immunotherapies.
Science. 2020 (a review/perspective article)PDF.
Precise T cell recognition programs designed by transcriptionally linking multiple receptors.
Engineering synthetic morphogen systems that can program multicellular patterning.
Discriminatory Power of Combinatorial Antigen Recognition in Cancer T Cell Therapies.
Cell Systems. 2020Antigen explorer webserver. PDF.
Engineering T cells to Treat Cancer: The Convergence of Immuno-Oncology and Synthetic Biology
The Annual Review of Cancer Biology. 2020PDF.
Engineering cell–cell communication networks: programming multicellular behaviors.
Current Opinion in Chemical Biology. 2019 (a review article)PDF.
Simple Rules Determine Distinct Patterns of Branching Morphogenesis.
Cell Systems. 2019PDF.
The Design Principles of Biochemical Timers: Circuits that Discriminate between Transient and Sustained Stimulation.
Cell Systems. 2019PDF.
Synthetic development: learning to program multicellular self-organization.
Current Opinion in Systems Biology . 2019 (a review article)PDF.
Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway.
Science. 2018 Aug 31PDF.
High-throughput multicolor optogenetics in microwell plates.
Nature Protocols. 2019PDF.
Programming self-organizing multicellular structures with synthetic cell-cell signaling.
Science. 2018 May 31PDF.
Building a Stable Relationship: Ensuring Homeostasis among Cell Types within a Tissue.
Cell. 2018 May 31PDF.
Synthetic Immunology: Hacking Immune Cells to Expand Their Therapeutic Capabilities.
Annu Rev Immunol. 2017 May 31PDF.
Design of Tunable Oscillatory Dynamics in a Synthetic NF-kB Signaling Circuit.
Cell Syst.. 2017 Nov 22PDF.
Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control.
Mol Cell. 2017 Sep 7PDF.
Engineering Therapeutic T cells: From Synthetic Biology to Clinical Trials.
Annu Rev Pathol. 2017 JanPDF.
Interrogating cellular perception and decision making with optogenetic tools.
J Cell Biol. 2017 April 7PDF.
CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells.
Scientific Reports. 2017 April 7PDF.
The Principles of Engineering Immune Cells to Treat Cancer.
Cell. 2017 February 9PDF.
Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Nat Methods. 2016 DecemberPDF.
Engineering dynamical control of cell fate switching using synthetic phospho-regulons.
Proceedings of the National Academy of Sciences. 2016 November 22PDF.
Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of mutiple genomic loci.
Nucleic Acids Research. 2016 January 5PDF.
Engineering T cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors.
Cell. 2016 October 6PDF.
Cellular perception and misperception: Internal models for decision-making shaped by evolutionary experience.
Bioessays. 2016 July 27PDF.
Modular engineering of cellular signaling proteins and networks.
Curr Opin Struct Biol. 2016 July 13PDF.
CRISPR/Cas9 for Human Genome Engineering and Disease Research.
Annu Rev Genomics Hum Genet. 2016 May 23PDF.
Nucleosome breathing and remodeling constrain CRISPR-Cas9 function.
eLife. 2016; 5:e13450 Apr 28PDF.
Precision tumor recognition by T cells with combinatorial antigen-sensing circuits.
Cell. 2016 Feb. 11PDF. Supplemental Info.
Engineering customized cell sensing and response behaviors using synthetic notch receptors.
Cell. 2016 Feb. 11PDF. Supplemental Info. Addgene.
*These authors contributed equally to this work.
Mapping the functional versatility and fragility of Ras GTPase signaling circuits through in vitro network reconstitution.
eLife. 2016;5:12435 Jan. 14PDF.
Beyond editing: Repurposing CRISPR-Cas9 for precision genome regulation and interrogation
Nature Reviews Molecular Cell Biology. 2015 Dec 16PDF.
Oscillatory stress stimulation uncovers an Achilles' heel of the yeast MAPK signaling network
Science. 2015 Nov 19PDF.
Remote control of therapeutic T cells through a small molecule-gated chimeric receptor
Science. 2015 Sep 24PDF.
Differential genetic interactions of yeast stress response MAPK pathways
Mol Syst Biol. 2015 Apr 17PDF.
Synthetic biology approaches to engineer T cells
Current Opinion in Immunology. 2015; 35:123-130PDF.
Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds
Cell. 2015 Jan 15;160(1-2):339-50.PDF.
Specific Gene Repression by CRISPRi System Transferred through Bacterial Conjugation
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CRISPR interference (CRISPRi) for sequence-specific control of gene expression.
Nature Protocols. 2013 Nov 8.PDF.
Genetic sensor for strong methylating compounds.
ACS Synth Biol. 2013 Oct 18.PDF.
Actin dynamics rapidly reset chemoattractant receptor sensitivity following adaptation in neutrophils.
Philos Trans R Soc Lond B Biol Sci. 2013 Sept 23.PDF.
Sending mixed messages for cell population control.
Cell (Preview) 158 : 973-975 (2014) (PDF)
Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal.
Proceedings of the National Academy of Sciences (April 2014)
Secreting and sensing the same molecule allows cells to achieve versatile social behaviors.
Science 343 (6171): 1242782 (2014)
Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module.
Cell 155(6):1422-34 (2013)
Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory.
Proceedings of the National Academy of Sciences 110(40):16015-16020 (2013)
Exploitation of Latent Allostery Enables the Evolution of New Modes of MAP Kinase Regulation.
Cell 154, 875-887 (2013)
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.
Cell (Resource) 154, 1-10 (2013)
Cell-based therapeutics: the next pillar of medicine.
Science Translational Medicine 5(179) (2013)
Cell biology 2.0.
Trends Cell Biol. 22(12):611-2 (2013)
Design principles of regulatory networks: searching for the molecular algorithms of the cell.
Mol. Cell 49(2):202-12 (2013)
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Cell 28;152(5):1173-83 (2013)
Designing synthetic regulatory networks capable of self-organizing cell polarization.
Cell 151(2):320-32 (2012)
Conformational Control of the Ste5 Scaffold Protein Insulates Against MAP Kinase Misactivation.
Science 337 (6099): 1218-1222 (2012)
Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells.
Nature 488, 384-388 (2012)
Systematic functional prioritization of protein posttranslational modifications.
Cell 150(2):413-25 (2012)
SYNZIP Protein Interaction Toolbox: in Vitro and in Vivo Specifications of Heterospecific Coiled-Coil Interaction Domains.
ACS Synth Biol. 1(4): 118-129 (2012)
Control of protein signaling using a computationally designed GTPase/GEF orthogonal pair.
Proceedings of the National Academy of Sciences 109(14): 5277-5282 (2012)
Light-based feedback for controlling intracellular signaling dynamics
Nature Methods 8, 837-839 (2011)
Recruitment interactions can override catalytic interactions in determining the functional identity of a protein kinase
Proceedings of the National Academy of Sciences 108, 9809-9814 (2011)
Light Control of Plasma Membrane Recruitment Using the Phy-PIF System
Methods Enzymology 497, 409-23 (2011)
Scaffold proteins: hubs for controlling the flow of cellular information
Science 332, 680-6 (2011)
The promise of optogenetics in cell biology: interrogating molecular circuits in space and time
Nature Methods 1, 35-38 (2011)
Build life to understand it
Nature 468, 889-890 (2010)
Phosphotyrosine signaling: evolving a new cellular communication system
Cell 142, 661-667 (2010)
The minimal autoinhibited unit of the guanine nucleotide exchange factor intersectin
PLos ONE 5, e11291 (2010)
Designing customized cell signalling circuits
Nature Reviews Molecular Cell Biology 11, 393-403 (2010)
Rapid diversification of cell signaling phenotypes by modular domain recombination
Science 328, 368-372 (2010)
Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systems
Annual Review of Biophysics 39, 515-537 (2010)
Reengineering the signaling properties of a Src family kinase
Biochemistry 48, 10956-10962 (2009)
Spatiotemporal control of cell signalling using a light-switchable protein interaction
Nature 461, 997-1001 (2009)
Defining network topologies that can achieve biochemical adaptation
Cell 138, 760-73 (2009)
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The Ste5 Scaffold Directs Mating Signaling by Catalytically Unlocking the Fus3 MAP Kinase for Activation
Cell 136, 1085-97 (2009)
The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency
Nature 454, 1005-8 (2008)
Evolution of the phospho-tyrosine signaling machinery in premetazoan lineages
Proceedings of the National Academy of Sciences 105, 9680-4 (2008)
Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics
Science 319, 1539-43 (2008)
Synthetic biology: lessons from the history of synthetic organic chemistry
Nature Chemical Biology 3, 521-5 (2007)
Rewiring cellular morphology pathways with synthetic guanine nucleotide exchange factors
Nature 447, 596-600 (2007)
Engineering synthetic signaling proteins with ultrasensitive input/output control
Nature Biotechnology 25, 660-662 (2007)
Engineering modular protein interaction switches by sequence overlap
Journal of the American Chemical Society 129, 4606-4611 (2007)
Docking interactions in protein kinase and phosphatase networks
Current Opinion in Structural Biology 16, 676-685 (2006)
Domains, Motifs, and Scaffolds: The Role of Modular Interactions in the Evolution and Wiring of Cell Signaling Circuits
Annual Review of Biochemistry 75, 655-680 (2006)
The Ste5 Scaffold Allosterically Modulates Signaling Output of the Yeast Mating Pathway
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The role of docking interactions in mediating discrimination in the yeast MAPK network
Molecular Cell 20, 951-962 (2005)
A general model for preferential hetero-oligomerization of Lin-2/7 domains: Mechanism underlying directed assembly of supramolecular signaling complexes
Journal of Biological Chemistry 280, 38528-38536 (2005)
A Polybasic Motif Allows N-WASP to Act as a Sensor of PIP2 Density
Molecular Cell 17, 181-191 (2005)
Rewiring cell signaling: the logic and plasticity of eukaryotic protein circuitry
Current Opinion in Structural Biology 14, 690-699 (2004)
Sho1 and Pbs2 Act as Coscaffolds Linking Components in the Yeast High Osmolarity MAP Kinase Pathway
Molecular Cell 14, 825–832 (2004)
Optimization of specificity in a cellular protein interaction network by negative selection
Nature 426, 676-680 (2003)
Reprogramming Control of an Allosteric Signaling Switch Through Modular Recombination
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The Structure and Function of Proline Recognition Domains
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Biochemistry 42, 2797-2805 (2003)
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Cell 111, 565–576 (2002)
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Journal of Biological Chemistry 277, 34902-34908 (2002)
How signaling proteins integrate multiple inputs: a comparison of N-WASP and Cdk2
Current Opinion in Cell Biology14, 149-154 (2002)
The modular logic of signaling proteins: building allosteric switches from simple binding domains
Current Opinion in Structural Biology 12, 61-68 (2002)
Structure of the SH3-Guanylate Kinase Module from PSD-95 Suggests a Mechanism for Regulated Assembly of MAGUK Scaffolding Proteins
Molecular Cell 8, 1291-1301 (2001)
Mechanism and role of PDZ domains in signaling complex assembly
Journal of Cell Science 114, 3219-3231 (2001)
The double life of PX domains
Nature Structural Biology 8, 570-572 (2001)
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Biochemistry 40, 5921-5930 (2001)
Integration of Multiple Signals Through Cooperative Regulation of the N-WASP-Arp2/3 Complex
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Converging on proline: the mechanism of WW domain peptide recognition
Nature Structural Biology 7, 611-613 (2000)
Improving SH3 domain ligand selectivity using a non-natural scaffold
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PSD-95 Assembles a Ternary Complex with the N-Methyl-D-aspartic Acid Receptor and a Bivalent Neuronal NO Synthase PDZ Domain
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Structure of the Enabled/VASP Homology 1 DomainPeptide Complex: A Key Component in the Spatial Control of Actin Assembly
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Unexpected Modes of PDZ Domain Scaffolding Revealed by Structure of nNOS-Syntrophin Complex
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