CAR-T Cell-Mediated B-Cell Depletion in Central Nervous System Autoimmunity
Neurol Neuroimmunol Neuroinflamm. 2023PDF.
Instructional materials that control cellular activity through synthetic Notch receptors
Harnessing synthetic biology to engineer organoids and tissues
Cell Stem Cell. 2023PDF.
Synthetic cytokine circuits that drive T cells into immune-excluded tumors
Programming Multicellular Assembly with Synthetic Cell Adhesion Molecules
Nature. 2022PDF. UCSF Press Release.
Decoding CAR T cell phenotype using combinatorial signaling motif libraries and machine learning
Science. 2022PDF. UCSF Press Release.
The emerging era of cell engineering: Harnessing the modularity of cells to program complex biological function
Rethinking cancer targeting strategies in the era of smart cell therapeutics
Nat Rev Cancer. 2022PDF.
SynNotch-CAR T cells overcome challenges of specificity, heterogeneity, and persistence in treating glioblastoma
Sci Transl Med. 2021PDF. PRESS UCSF.
T cell circuits that sense antigen density with an ultrasensitive threshold
Science. 2021PDF. FEATURED IN Cell Research. FEATURED IN Signal Transduction and Targeted Therapy
T cells selectively filter oscillatory signals on the minutes timescale
Proc Natl Acad Sci U S A. 2021PDF.
DNA scaffolds enable efficient and tunable functionalization of biomaterials for immune cell modulation
Nat Nanotechnol. 2021PDF.
Precise T cell recognition programs designed by transcriptionally linking multiple receptors
Engineering cytokines and cytokine circuits
Engineering synthetic morphogen systems that can program multicellular patterning
Discriminatory Power of Combinatorial Antigen Recognition in Cancer T Cell Therapies
Cell Syst. 2020PDF.
Simple Rules Determine Distinct Patterns of Branching Morphogenesis
Cell Syst. 2019PDF.
The Design Principles of Biochemical Timers: Circuits that Discriminate between Transient and Sustained Stimulation
Cell Syst. 2019PDF.
High-throughput multicolor optogenetics in microwell plates
Nat Protoc. 2019PDF.
Engineering cell-cell communication networks: programming multicellular behaviors
Curr Opin Chem Biol. 2019PDF.
Programming self-organizing multicellular structures with synthetic cell-cell signaling
Building a Stable Relationship: Ensuring Homeostasis among Cell Types within a Tissue
Design of Tunable Oscillatory Dynamics in a Synthetic NF-κB Signaling Circuit
Cell Syst. 2017PDF.
Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control
Mol Cell. 2017PDF.
Synthetic Immunology: Hacking Immune Cells to Expand Their Therapeutic Capabilities
Annu Rev Immunol. 2017PDF.
CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells
Sci Rep. 2017PDF.
The Principles of Engineering Immune Cells to Treat Cancer
Interrogating cellular perception and decision making with optogenetic tools
J Cell Biol. 2017PDF.
Engineering Therapeutic T Cells: From Synthetic Biology to Clinical Trials
Annu Rev Pathol. 2017PDF.
Engineering dynamical control of cell fate switching using synthetic phospho-regulons
Proc Natl Acad Sci U S A. 2016PDF.
Complex transcriptional modulation with orthogonal and inducible dCas9 regulators
Nat Methods. 2016PDF.
Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors
Cellular perception and misperception: Internal models for decision-making shaped by evolutionary experience
Modular engineering of cellular signaling proteins and networks
Curr Opin Struct Biol. 2016PDF.
CRISPR/Cas9 for Human Genome Engineering and Disease Research
Annu Rev Genomics Hum Genet. 2016PDF.
Nucleosome breathing and remodeling constrain CRISPR-Cas9 function
Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits
Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors
Mapping the functional versatility and fragility of Ras GTPase signaling circuits through in vitro network reconstitution
Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci
Nucleic Acids Res. 2016PDF.
Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation
Nat Rev Mol Cell Biol. 2016PDF.
Oscillatory stress stimulation uncovers an Achilles' heel of the yeast MAPK signaling network
Remote control of therapeutic T cells through a small molecule-gated chimeric receptor
Synthetic biology approaches to engineer T cells
Curr Opin Immunol. 2015PDF.
Differential genetic interactions of yeast stress response MAPK pathways
Mol Syst Biol. 2015PDF.
Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds
Specific gene repression by CRISPRi system transferred through bacterial conjugation
ACS Synth Biol. 2014PDF.
Sending mixed messages for cell population control
Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal
Proc Natl Acad Sci U S A. 2014PDF.
Secreting and sensing the same molecule allows cells to achieve versatile social behaviors
Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module
CRISPR interference (CRISPRi) for sequence-specific control of gene expression
Nat Protoc. 2013PDF.
Actin dynamics rapidly reset chemoattractant receptor sensitivity following adaptation in neutrophils
Philos Trans R Soc Lond B Biol Sci. 2013PDF.
Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory
Proc Natl Acad Sci U S A. 2013PDF.
Exploitation of latent allostery enables the evolution of new modes of MAP kinase regulation
CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes
Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae
PLoS One. 2013PDF.
Cell-based therapeutics: the next pillar of medicine
Sci Transl Med. 2013PDF.
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression
Bridging cross-cultural gaps in scientific exchange through innovative team challenge workshops
Quant Biol. 2013PDF.
Design principles of regulatory networks: searching for the molecular algorithms of the cell
Mol Cell. 2013PDF.
Designing synthetic regulatory networks capable of self-organizing cell polarization
Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation
Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells
Systematic functional prioritization of protein posttranslational modifications
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Proc Natl Acad Sci U S A. 2012PDF.
Light-based feedback for controlling intracellular signaling dynamics
Nat Methods. 2011PDF.
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Proc Natl Acad Sci U S A. 2011PDF.
Light control of plasma membrane recruitment using the Phy-PIF system
Methods Enzymol. 2011PDF.
Scaffold proteins: hubs for controlling the flow of cellular information
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Nat Methods. 2011PDF.
Phosphotyrosine signaling: evolving a new cellular communication system
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PLoS One. 2010PDF.
Designing customized cell signalling circuits
Nat Rev Mol Cell Biol. 2010PDF.
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Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systems
Annu Rev Biophys. 2010PDF.
Spatiotemporal control of cell signalling using a light-switchable protein interaction
Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species
PLoS Biol. 2009PDF.
The Ste5 scaffold directs mating signaling by catalytically unlocking the Fus3 MAP kinase for activation
Frederic M Richards 1925-2009
Nat Struct Mol Biol. 2009PDF.
The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency
Evolution of the phospho-tyrosine signaling machinery in premetazoan lineages
Proc Natl Acad Sci U S A. 2008PDF.
Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics
Synthetic biology: lessons from the history of synthetic organic chemistry
Nat Chem Biol. 2007PDF.
Rewiring cellular morphology pathways with synthetic guanine nucleotide exchange factors
Engineering synthetic signaling proteins with ultrasensitive input/output control
Nat Biotechnol. 2007PDF.
Engineering modular protein interaction switches by sequence overlap
J Am Chem Soc. 2007PDF.
Docking interactions in protein kinase and phosphatase networks
Curr Opin Struct Biol. 2006PDF.
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Annu Rev Biochem. 2006PDF.
The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway
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Mol Cell. 2005PDF.
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J Biol Chem. 2005PDF.
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Mol Cell. 2005PDF.
Rewiring cell signaling: the logic and plasticity of eukaryotic protein circuitry
Curr Opin Struct Biol. 2004PDF.
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Mol Cell. 2004PDF.
Optimization of specificity in a cellular protein interaction network by negative selection
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The structure and function of proline recognition domains
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Complex inheritance of familial hypercholanemia with associated mutations in TJP2 and BAAT
Nat Genet. 2003PDF.
Role of electrostatic interactions in PDZ domain ligand recognition
Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms
Shared receptors in axon guidance: SAX-3/Robo signals via UNC-34/Enabled and a Netrin-independent UNC-40/DCC function
Nat Neurosci. 2002PDF.
Coordinated folding and association of the LIN-2, -7 (L27) domain. An obligate heterodimerization involved in assembly of signaling and cell polarity complexes
J Biol Chem. 2002PDF.
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Curr Opin Struct Biol. 2002PDF.
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Mol Cell. 2001PDF.
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An analysis of the interactions between the Sem-5 SH3 domain and its ligands using molecular dynamics, free energy calculations, and sequence analysis
J Am Chem Soc. 2001PDF.
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Chem Biol. 2000PDF.
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