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Light-based feedback for controlling intracellular signaling dynamics

Jared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. Weiner

Nature Methods 8, 837-839 (2011)

Matlab toolbox for microscope control (via Micro-Manager) and the feedback controller


Scaffold proteins: hubs for controlling the flow of cellular information

Matthew C. Good, Jesse G. Zalatan, Wendell A. Lim

Science 332, 680-6 (2011)

Mechanisms of scaffold protein Ste5
(animation by Janet Iwasa )

Quicktime video

Windows Media video


MPEG-4 video


Spatiotemporal control of cell signalling using a light-switchable protein interaction

Anselm Levskaya, Orion D. Weiner, Wendell A. Lim, and Christopher A. Voigt

Nature 461, 997-1001 (2009)

Supplementary Movies File

Movie S1 - YFP memane recruitment

Movie S2 - YFP memane dissociation

Movie S3 - oscillating YFP translocation

Movie S4 - YFP point recruitment

Movie S5 - patterned recruitment of YFP

Movie S6 - titrated recruitment of YFP

Movie S7 - cell extrusion by Tiam (Rac GEF) recruitment

Movie S8 - dynamic lamellipodia by Tiam (Rac GEF) recruitment

Movie S9 - periodic contractions by Tim (Rho GEF) recruitment

Movie S10 - PIF-YFP-only recruitment control

Movie S11 - dynamics of memane-bound Cdc42-GTP in TIRF


The Ste5 Scaffold Directs Mating Signaling by Catalytically Unlocking the Fus3 MAP Kinase for Activation

Matthew Good, Grace Tang, Julie Singleton, Attila Reményi, and Wendell A. Lim

Cell 136, 1085-97 (2009)

Structure File

'Minimal scaffold' (ms) domain of Ste5


The Ste5 Scaffold Allosterically Modulates Signaling Output of the YeastMating Pathway

Roby P. Bhattacharyya*, Attila Reményi*,Matthew C. Good, Caleb J. Bashor, Arnold M. Falick, and Wendell A. Lim

Science 311, 822-826 (2006)

Structure File

Fus3 in complex with a Ste5 peptide


Fus3 without a peptide from Ste5


Fus3 phosphorylated on Tyr182


The role of docking interactions in mediating signaling input, output, anddiscrimination in the yeast MAPK network

AttilaReményi, Matthew C. Good, Roby P. Bhattacharyya, andWendell A. Lim

Molecular Cell 20, 951-962 (2005)

Structure File

Mitogen activated protein kinase Fus3 (non-phosphorylated)


Fus3 with a docking motif from Ste7


Fus3 with a docking motif from Msg5


Fus3 with a docking motif from Far1


Structure of the N-WASP EVH1 Domain-WIP Complex: Insight into the Molecular Basisof Wiskott-Aldrich Syndrome

ian F. Volkman, Kenneth E. Prehoda, Jessica A.Scott, Francis C. Peterson, and Wendell A. Lim

Cell 111, 565–576 (2002)

Structure File

N-WASP EVH1 Domain - WIP complex


Structure of the SH3-Guanylate Kinase Module from PSD-95 Suggests a Mechanism for Regulated Assembly of MAGUK Scaffolding Proteins

Aaron W. McGee, Srikanth R. Dakoji, Olav Olsen, David S. edt, Wendell A. Lim, and Kenneth E. Prehoda

Molecular Cell 8, 1291-1301 (2001)

Structure File

SH3-guanylate kinase module from PSD-95


Structure of the Enabled/VASP Homology 1 Domain–Peptide Complex: A Key Component in the Spatial Control of Actin Assembly

Kenneth E. Prehoda, Do. J. Lee, and Wendell A. Lim

Cell 97, 471-480 (1999)

Structure File

Enabled/VASP Homology 1 (EVH1) domain from mouse Enabled in complex with ActA peptide


Unexpected Modes of PDZ Domain Scaffolding Revealed by Structure of nNOS-SyntrophinComplex

ian J. Hillier, Karen S. Christopherson, Kenneth E. Prehoda, David S. edt, and Wendell A. Lim

Science 284, 812-815 (1999)

Structure File

Neuronal nitric oxide synthase (nNOS) PDZ domain


nNOS PDZ-syntrophin PDZ complex


Exploiting the Basis of Proline Recognition by SH3 andWW Domains: Design of N-Substituted Inhibitors

Jack T. Nguyen, Christoph W. Turck, Fred E. Cohen, Ronald N. Zuckermann, and Wendell A. Lim

Science 282, 2088-2092 (1998)

Structure File

Sem5 C-term. SH3 in complex with Sos peptide


Sem5 C-term. SH3 in complex with isopropyl peptoid/peptide


Sem5 C-term. SH3 in complex with cyclopropyl methyl peptoid/peptide


Crk SH3 in complex with peptoid/peptide