Software

Light-based feedback for controlling intracellular signaling dynamics
Jared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. Weiner
Nature Methods 8, 837-839 (2011)

Matlab toolbox for microscope control (via Micro-Manager) and the feedback controller

software.zip



Movies

Scaffold proteins: hubs for controlling the flow of cellular information
Matthew C. Good, Jesse G. Zalatan, Wendell A. Lim
Science 332, 680-6 (2011)

Mechanisms of scaffold protein Ste5
(animation by Janet Iwasa)

File

Quicktime video

matingPathway.mov

Windows Media video

matingPathway.wmv

MPEG-4 video

matingPathway.mp4



Spatiotemporal control of cell signalling using a light-switchable protein interaction
Anselm Levskaya, Orion D. Weiner, Wendell A. Lim, and Christopher A. Voigt
Nature 461, 997-1001 (2009)

Supplementary Movies

File

Movie S1 - YFP membrane recruitment

al_2009_s1.mov

Movie S2 - YFP membrane dissociation

al_2009_s2.mov

Movie S3 - oscillating YFP translocation

al_2009_s3.mov

Movie S4 - YFP point recruitment

al_2009_s4.mov

Movie S5 - patterned recruitment of YFP

al_2009_s5.mov

Movie S6 - titrated recruitment of YFP

al_2009_s6.mov

Movie S7 - cell extrusion by Tiam (Rac GEF) recruitment

al_2009_s7.mov

Movie S8 - dynamic lamellipodia by Tiam (Rac GEF) recruitment

al_2009_s8.mov

Movie S9 - periodic contractions by Tim (Rho GEF) recruitment

al_2009_s9.mov

Movie S10 - PIF-YFP-only recruitment control

al_2009_s10.mov

Movie S11 - dynamics of membrane-bound Cdc42-GTP in TIRF

al_2009_s11.mov



Structures

The Ste5 Scaffold Directs Mating Signaling by Catalytically Unlocking the Fus3 MAP Kinase for Activation
Matthew Good, Grace Tang, Julie Singleton, Attila Reményi, and Wendell A. Lim
Cell 136, 1085-97 (2009)

Structure

File

'Minimal scaffold' (ms) domain of Ste5

3FZE.pdb



The Ste5 Scaffold Allosterically Modulates Signaling Output of the Yeast Mating Pathway
Roby P. Bhattacharyya*, Attila Reményi*, Matthew C. Good, Caleb J. Bashor, Arnold M. Falick, and Wendell A. Lim
Science 311, 822-826 (2006)

Structure

File

Fus3 in complex with a Ste5 peptide

2F49.pdb

Fus3 without a peptide from Ste5

2FA2.pdb

Fus3 phosphorylated on Tyr182

2F9G.pdb



The role of docking interactions in mediating signaling input, output, and discrimination in the yeast MAPK network
Attila Reményi, Matthew C. Good, Roby P. Bhattacharyya, and Wendell A. Lim
Molecular Cell 20, 951-962 (2005)

Structure

File

Mitogen activated protein kinase Fus3 (non-phosphorylated)

2B9F.pdb

Fus3 with a docking motif from Ste7

2B9H.pdb

Fus3 with a docking motif from Msg5

2B9I.pdb

Fus3 with a docking motif from Far1

2B9J.pdb



Structure of the N-WASP EVH1 Domain-WIP Complex: Insight into the Molecular Basis of Wiskott-Aldrich Syndrome
Brian F. Volkman, Kenneth E. Prehoda, Jessica A. Scott, Francis C. Peterson, and Wendell A. Lim
Cell 111, 565–576 (2002)

Structure

File

N-WASP EVH1 Domain - WIP complex

1MKE.pdb



Structure of the SH3-Guanylate Kinase Module from PSD-95 Suggests a Mechanism for Regulated Assembly of MAGUK Scaffolding Proteins
Aaron W. McGee, Srikanth R. Dakoji, Olav Olsen, David S. Bredt, Wendell A. Lim, and Kenneth E. Prehoda
Molecular Cell 8, 1291-1301 (2001)

Structure

File

SH3-guanylate kinase module from PSD-95

1KJW.pdb



Structure of the Enabled/VASP Homology 1 Domain–Peptide Complex: A Key Component in the Spatial Control of Actin Assembly
Kenneth E. Prehoda, Do. J. Lee, and Wendell A. Lim
Cell 97, 471-480 (1999)

Structure

File

Enabled/VASP Homology 1 (EVH1) domain from mouse Enabled in complex with ActA peptide

1EVH.pdb



Unexpected Modes of PDZ Domain Scaffolding Revealed by Structure of nNOS-Syntrophin Complex
Brian J. Hillier, Karen S. Christopherson, Kenneth E. Prehoda, David S. Bredt, and Wendell A. Lim
Science 284, 812-815 (1999)

Structure

File

Neuronal nitric oxide synthase (nNOS) PDZ domain

1QAU.pdb

nNOS PDZ-syntrophin PDZ complex

1QAV.pdb



Exploiting the Basis of Proline Recognition by SH3 and WW Domains: Design of N-Substituted Inhibitors
Jack T. Nguyen, Christoph W. Turck, Fred E. Cohen, Ronald N. Zuckermann, and Wendell A. Lim
Science 282, 2088-2092 (1998)

Structure

File

Sem5 C-term. SH3 in complex with Sos peptide

1SEM.pdb

Sem5 C-term. SH3 in complex with isopropyl peptoid/peptide

2SEM.pdb

Sem5 C-term. SH3 in complex with cyclopropyl methyl peptoid/peptide

3SEM.pdb

Crk SH3 in complex with peptoid/peptide

1B07.pdb