Lim Lab | Papers

Engineering synthetic signaling proteins with ultrasensitive input/output control

John E. Dueber, Ethan A. Mirsky, and Wendell A. Lim
Nature Biotechnology 25, 660-662 (2007)

Many signaling proteins are built from simple, modular components, yet display highly complex signal-processing behavior. Here we explore how modular domains can be used to build an ultrasensitive switch-a nonlinear input/output function that is central to many complex biological behaviors. By systematically altering the number and affinity of modular autoinhibitory interactions, we show that we can predictably convert a simple linear signaling protein into an ultrasensitive switch.

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