elias    |    PUCHNER

elias.puchner ucsf.edu

Assistant Professor of Physics - University of Minnesota (Starting: January 2015)

Postdoctoral Fellow


Postdoctoral Fellow
University of California, San Francisco

2008 - 2009
Postdoctoral Fellow
Gaub lab, University of Munich

2006 - 2008
PhD, Physics and Biophysics
Gaub lab, University of Munich

2000 - 2006
Diploma, Physics and Biophysics with minor in Biochemistry
University of Munich

Three-month Internship
Fernandez lab, Columbia University, NY

Fellowships and Awards

2010-2012 Research fellowship of the Deutsche Forschungsgemeinschaft
2009 Publication prize, CENS (Center for Nanoscience, Munich)
2008 Attocube Wittenstein research award
2008 Publication prize, CENS (Center for Nanoscience, Munich)
2007-2008 Fellow of the International Doctorate Program NanoBio Technology
2007 Poster prize, European Biophysical Society, London


Investigating the spatial organization of pathway components and creating an artificial surface receptor with engineered input control.


Puchner, E.M., Walter, J.M., Kasper, R., Huang, B. and Lim, W.A. "Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory." Proceedings of the National Academy of Sciences 110(40):16015-16020 (2013).

Puchner, E.M. and H.E. Gaub "Single-Molecule Mechanoenzymatics." Annual Review of Biophysics 41, in press. (2012).

Stahl, S.W., E.M. Puchner, A. Alexandrovich, M. Gautel, and H.E. Gaub "A conditional gating mechanism assures the integrity of the molecular force-sensor titin kinase." Biophysical Journal 101, 1978-86. (2011).

Heus, H.A., E.M. Puchner, A.J. van Vugt-Jonker, J.L. Zimmermann, and H.E. Gaub "Atomic force microscope-based single-molecule force spectroscopy of RNA unfolding." Analytical Biochemistry 414, 1-6. (2011).

Puchner, E.M. and H.E. Gaub, "Mechanoenzymatics and Nanoassembly of Single Molecules", in Single Molecule Spectroscopy in Chemistry, Physics and Biology, Springer Series in Chemical Physics, Volume 96, Part 6, 289-303. (2010)

Puchner, E.M. and H.E. Gaub "Exploring the conformation-regulated function of titin kinase by mechanical pump and probe experiments with single molecules." Angewandte Chemie 49, 1147-50. (2010).

Geisler, M., S. Xiao, E.M. Puchner, F. Grater, and T. Hugel "Controlling the Structure of Proteins at Surfaces." Journal of the American Chemical Society 132(48):17277-81 (2010).

Cordes, T., M. Strackharn, S.W. Stahl, W. Summerer, C. Steinhauer, C. Forthmann, E.M. Puchner, J. Vogelsang, H.E. Gaub, and P. Tinnefeld "Resolving single-molecule assembled patterns with superresolution blink-microscopy." Nano Letters 10, 645-51. (2010).

Stahl, S.W., E.M. Puchner, and H.E. Gaub "Photothermal cantilever actuation for fast single-molecule force spectroscopy." The Review of scientific instruments 80, 073702. (2009).

Puchner, E.M. and H.E. Gaub "Force and function: probing proteins with AFM-based force spectroscopy." Current Opinion in Structural Biology 19, 605-14. (2009).

Kufer, S.K., M. Strackharn, S.W. Stahl, H. Gumpp, E.M. Puchner, and H.E. Gaub "Optically monitoring the mechanical assembly of single molecules." Nature Nanotechnology 4, 45-9. (2009).

Gumpp, H., S.W. Stahl, M. Strackharn, E.M. Puchner, and H.E. Gaub "Ultrastable combined atomic force and total internal reflection fluorescence microscope." The Review of scientific instruments 80, 063704. (2009).

Gumpp, H., E.M. Puchner, J.L. Zimmermann, U. Gerland, H.E. Gaub, and K. Blank "Triggering enzymatic activity with force." Nano Letters 9, 3290-5. (2009).

Puchner, E.M., S.K. Kufer, M. Strackharn, S.W. Stahl, and H.E. Gaub "Nanoparticle self-assembly on a DNA-scaffold written by single-molecule cut-and-paste." Nano Letters 8, 3692-5. (2008).

Puchner, E.M., G. Franzen, M. Gautel, and H.E. Gaub "Comparing proteins by their unfolding pattern." Biophysical Journal 95, 426-34. (2008).

Puchner, E.M., A. Alexandrovich, A.L. Kho, U. Hensen, L.V. Schafer, B. Brandmeier, F. Grater, H. Grubmuller, H.E. Gaub, and M. Gautel "Mechanoenzymatics of titin kinase." Proceedings of the National Academy of Sciences of the United States of America 105, 13385-90. (2008).

Morfill, J., J. Neumann, K. Blank, U. Steinbach, E.M. Puchner, K.E. Gottschalk, and H.E. Gaub "Force-based analysis of multidimensional energy landscapes: application of dynamic force spectroscopy and steered molecular dynamics simulations to an antibody fragment-peptide complex." Journal of Molecular Biology 381, 1253-66. (2008).

Kufer, S.K., E.M. Puchner, H. Gumpp, T. Liedl, and H.E. Gaub "Single-molecule cut-and-paste surface assembly." Science 319, 594-6. (2008).