Sho1 and Pbs2 Act as Coscaffolds Linking Components in the Yeast High Osmolarity MAP Kinase Pathway

Ali Zarrinpar, Roby P. Bhattacharyya, M. Paige Nittler, and Wendell A. Lim
Molecular Cell 14, 825–832 (2004)

Plasmids Used in this Study.

Plasmid	Description	Origin	Selection
Sho1-GFP 316	Sho1 wild-type-GFP	CEN/ARS	URA3
Sho1-HA 316	Sho1 wild-type-HA3	CEN/ARS	URA3
∆N-GFP 316	Sho1 ∆(5­28)-GFP	CEN/ARS	URA3
∆N-HA 316	Sho1 ∆(5­28)-HA3	CEN/ARS	URA3
L1-GFP 316	Sho1 (S61G, F62S, P63G)-GFP	CEN/ARS	URA3
L1-HA 316	Sho1 (S61G, F62S, P63G)-HA3	CEN/ARS	URA3
L2-GFP 316	Sho1 (L88G, V89G, D90S, H91G, Y92G)-GFP	CEN/ARS	URA3
L2-HA 316	Sho1 (L88G, V89G, D90S, H91G, Y92G)-HA3	CEN/ARS	URA3
L3-GFP 316	Sho1 (P120S, K121G)-GFP	CEN/ARS	URA3
L3-HA 316	Sho1 (P120S, K121G)-HA3	CEN/ARS	URA3
W338-GFP 316	Sho1 W338F-GFP	CEN/ARS	URA3
W338-HA 316	Sho1 W338F-HA3	CEN/ARS	URA3
∆(334­367)-GFP 316	Sho1 ∆(334­367)-GFP	CEN/ARS	URA3
∆(334­367)-HA 316	Sho1 ∆(334­367)-HA3	CEN/ARS	URA3
∆(306­367)-GFP 316	Sho1 ∆(306­367)-GFP	CEN/ARS	URA3
∆(306­367)-HA 316	Sho1 ∆(306­367)-HA3	CEN/ARS	URA3
∆(172­298)-GFP 316	Sho1 ∆(172­298)-GFP	CEN/ARS	URA3
∆(172­298)-HA 316	Sho1 ∆(172­298)-HA3	CEN/ARS	URA3
∆(184­217)-GFP 316	Sho1 ∆(184­217)-GFP	CEN/ARS	URA3
∆(184­217)-HA 316	Sho1 ∆(184­217)-HA3	CEN/ARS	URA3
Sho1-C-tail-HA 316	Sho1 ∆(1­144)-HA3	CEN/ARS	URA3
Ste11-Myc 424	GAL1, Ste11-Myc13	2 µ	TRP1
Ste11-CA 316	Ste11 (S281D S285D T286D)-HA3	CEN/ARS	URA3
Ste11-NA 316	Ste11 (S281A S285A T286A)-HA3	CEN/ARS	URA3
Ste11 316	Ste11 wild-type-HA3	CEN/ARS	URA3

Strains Used in This Study.

Strain	Description	Reference
SO355	MATa ssk2∆ ssk22∆ sho1∆ Fus1-lacZ	O’Rourke and Herskowitz
SO356	MATa sho1∆ hog1∆ Fus1-lacZ	O’Rourke and Herskowitz
AZ113	MATa ssk2∆ ssk22∆ sho1∆ pbs2∆ Fus1-lacZ	this study
RB101	MATa ssk2∆ ssk22∆ ste11∆	this study
RB102	MATa ssk2∆ ssk22∆ ste11∆ sho1∆	this study

Abstract
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